Coding
fecA-prhA

Part:BBa_K367006:Design

Designed by: Fernando Govantes   Group: iGEM10_UPO-Sevilla   (2010-06-30)

fecA-prhA fusion coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 409
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion junction generated between N-terminal part of FecA and rest of PrhA. Stop codons TAATAA were introduced, and codon usage was adapted to E. coli's using Mr. Gene tools. Purchased from Mr. Gene

Source

fecA sequence comes from Escherichia coli str. K-12 substr. MG1655, complete genome. GenBank: U00096.2

prhA sequence comes from Ralstonia solanacearum GMI1000 plasmid pGMI1000MP, complete sequence. NCBI Reference Sequence: NC_003296.1

References